We want to modify a polymer surface by a another media with through covalent bonding. How we can to be sure for formation of covalent bonding in a reaction?
I am confused by your question. Do you want to be sure _before_ you do the reaction that it is a chemical reaction that happens via covalent bonding? Alternatively, do you want to be sure _after_ you do the reaction that you have modified the surface as per a proposed chemical reaction pathway?
In general, I would think that you should probably have some idea of what your reaction will be before you go about doing the modification. In other words, you should probably know the chemistry of your polymer surface and know what chemicals do and do not "react" with it. So, I might guess that what you are after is a confirmation that you have modified your surface as per your proposed chemical reaction. But, that might be a wrong guess.
we want a method or assay for creation of some covalent bonding like amino groups or etc. in other word we want a test to know about that creation through it
OK. You want to confirm that your surface has been modified. Suppose you have two samples, one before and one after you have functionalized the surface.
Presuming nothing to start ...
-- You can do spectroscopy (surface spectroscopy). The best would be x-ray photoelectron spectroscopy.
Presuming that you know the chemistry of your new functional group and what reacts with it instead of the untreated surface ...
-- You can do chemical titrations.
Presuming that your functionalized surface has a different polar or non-polar surface chemistry on it ...
-- You can do contact angle studies.
Finally, presuming that you know the covalent bond cannot be broken by water or some other solvent ...
-- You can do immersion studies to see what leaches off the surfaces.