We have different medicinal plant extracts. So please suggest the recent trends used for free radical scavenger activity.
Through the DPPH assay.
Take a look at this article: Influence of the drying method in the antioxidant potential and chemical composition of four shrubby flowering plants from the tribe Genisteae (Fabaceae)
Dear Dr Vinesh You could determine the free radical scavenging by different methods like ABTS, DPPH, reducing power etc.
This article may be interesting to you: A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives
Method for DPPH Free Radical Scavenging Activity
The antioxidant activities of plant extracts and the standard were assessed on the basis of the free radical scavenging effect of the stable 1,1-diphenyl-2-icrylhydrazyl (DPPH)-freeradical activity by modified method [5]. The diluted working solutions of the test extracts were prepared in methanol. Ascorbic acid (10mg/ml) was used as standard. 0.002% DPPH was prepared in methanol and 3 ml of this solution was mixed with 1 ml of sample solution and standard solution separately.These solution mixtures were kept in dark for 30 min and optical density was measured at 517 nm using Spectrophotometer. Methanol (1 ml) with DPPH solution (0.002%, 3 ml)
was used as blank. The optical density was recorded and % inhibition was calculated
using the formula given below: Absorbance (cont) - absorbance (sam)
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Absorbance (cont)
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