How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

Is there a faster way of removing and collecting the carbon nanotubes on the surface of the stainless steel 304 plates other than sonification?

The usual way of properly removing and collecting carbon nanotubes (CNT) is to sonicate the stainless steel plates coated with CNT under isopropyl alcohol (IPA) solution, remove the plates after...

28 January 2021 9,455 1 View

Can anyone suggest an RNA extraction protocol from grape berries?

I am trying to extract RNA, from grape berries of a red variety, for qPCR (to study gene expression). I have tried nucleozol and a CTAB protocol (with b-mercaptoethanol+boric acid + cleaning with...

18 January 2021 8,857 2 View

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16 January 2021 8,334 5 View

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture??

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture. I got poked after sprayed them with 70 % alcohol and dried them right away. Is...

07 January 2021 9,412 2 View