I am trying to synthesize chitosan - Au nanoparticles using plant extract. But I am unable to confirm the formation of nanoparticles. I tried with both type of chitosan - low and medium molecular weight. Medium molecular wt chitosan was unable to dissolve in 1% acetic acid solution. While low molecular wt did dissolved. I took 20ml, 0.1% chitosan in 1% acetic acid. Added 100microlit of Auric chloride (1mM) solution. Then added 6.5ml leaf extract dropwise with constant stirring for 30 min. But still I am unable to get peak in UV spectrophotometer. Can anyone suggest me alternate protocol or else tell me where I am going wrong?
Have you been able to produce nanoparticles without chitosan? If so it will probably be easier to first prepare the nanoparticles and then proceed to chitosan adsorption.
TPP normally is a good crosslinker to make chitosan nanoparticles. the chitosan would dissolve in acetic acid 1-2%, just give it enough time plus stirring and heating to 70-80C
This paper may help you.
http://www.tandfonline.com/doi/abs/10.1080/09205063.2017.1338501
@Omar... I have prepared Au nanoparticles (without using chitosan successfully). I guess i will try your suggestion. Thank you.
@Amin... I know about TPP. But it is used instead of leaf extract. And I want prepare Chitosan - Au nanoparticles using leaf extract.
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