I need help with docking a fatty acid and insulin receptor IR3, I have tried the docking and got an reference RMSD value of 38.59 and binding energy of -3.39. The exact step I have followed is
Adding Polar Hydrogen - Adding Kollmann Charges -Write PDB - Open Ligand -Detect root - Choose root - Save Ligand - Grid - Choose Macromolecule - Choose Ligand - Grid Box - Save gpf - Running Autogrid - Running Autodock using Genetic Algorithm and Lamarckian Algorithm
I have also tried minimizing the energy of the fatty acid using Avagadro.
Kindly help.