I have isolated bovine neutrophils and i was able to check its viability by trypan blue staining in haemocytometer. However, Inorder to check the purity of these neutrophils, i need a protocol. Pls help me with this..
I tried making a smear form final cell suspension, air dried and methanol fixed it. Stained with Giemsa stain for 1 hr. However, result was obsolate
Hello, have you tried with cytospin and Diff quick staining? That technique is easy, quick and usually works all the time. To check the purity you need to do it by flow cytometry as well to confirm the percentage and purity of your population. You can use CD11b+ Gr-1+ cells to confirm they are neutrophils.
use May-Grunwald stain for 2 minutes, remove May-Grunwald stain without washing then add Giemsa stain for 1-2 minutes then wash and check it..
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