Hi guys, I am doing experiment of stereotactic intracranial surgery. I wander how to adjust the orientation of rat head in stereotactic to make sure I can injection drug precisely into the relative small area in rat brain as indicated in Paxinos rat atlas?
Thank you very much!
Hi Wenchong,
It depends on what animal you are using , body weight and what target in the brain you are aiming for, But, in general the standard way of head positioning in a Kopf stereotaxic instrument, for adult male rat is done so to keep the heights of Lambda & Bregma equal. and the line passing between the incisor bar in the horizontal plane through the interaural line , and the line between the Lambda and interaural bar in the lateral plane are making a 90 degree angle , (i.e.) the result is a flat skull position with straight line falling from Lambda trough the ear . accordingly in order to achieve this you need to lower the incisor bar 3. 0 mm below horizontal zero (-3 mm). and according to The Rat Brain in Stereotaxic Coordinates, 4th Edition by George Paxinos (1998) | ISBN: 0125476175, in Male wistar 290 gm rat, the incisor bar is lowered to 3.3 ± 0.4 mm below the horizontal zero.
if you are working with different strain of rats and different body weight , and aiming for deeper areas in the brain like VMH or Nucleus Tractus Solitarius, you need to calibrate and adjust your measurements accordingly.
"please see schematics below"
hope this is helping,
kind regards,
Ghanim.
Hi Wenchong,
It depends on what animal you are using , body weight and what target in the brain you are aiming for, But, in general the standard way of head positioning in a Kopf stereotaxic instrument, for adult male rat is done so to keep the heights of Lambda & Bregma equal. and the line passing between the incisor bar in the horizontal plane through the interaural line , and the line between the Lambda and interaural bar in the lateral plane are making a 90 degree angle , (i.e.) the result is a flat skull position with straight line falling from Lambda trough the ear . accordingly in order to achieve this you need to lower the incisor bar 3. 0 mm below horizontal zero (-3 mm). and according to The Rat Brain in Stereotaxic Coordinates, 4th Edition by George Paxinos (1998) | ISBN: 0125476175, in Male wistar 290 gm rat, the incisor bar is lowered to 3.3 ± 0.4 mm below the horizontal zero.
if you are working with different strain of rats and different body weight , and aiming for deeper areas in the brain like VMH or Nucleus Tractus Solitarius, you need to calibrate and adjust your measurements accordingly.
"please see schematics below"
hope this is helping,
kind regards,
Ghanim.
Take a look at this link for some basic information.
https://www.urmc.rochester.edu/neurosurgery/for-patients/treatments/stereotactic-brain-surgery.aspx
I suggest you use an anatomical atlas for rodents. I suggest the one published by Paxino and Watson.
There are some basic tutorial for rodents stereotactic brain surgery in youtube.
Good luck,
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