Hi all,
I was wondering if there is a way to separate oomycete RNA from plant RNA?
Any thoughts or suggestions are welcomed.
Thanks!
Hi everyone, I have a question about pooling libraries for RNAseq. I have 12 samples that I would like to run RNAseq with. Samples 1-6 were extracted from pure fungal cultures, however, samples...
01 February 2019 3,585 5 View
Hi All, I was trying to analyze some counting data using proc glimmix. I did have a lot of zeros in my counting data, and I specified that the distribution of the counting data follows negative...
05 June 2017 9,964 4 View
Hi All, I was wondering How many fungal isolates are needed to present the whole population phenotypically and genotypically in general? How can I test the numbers of the isolates used are large...
31 December 2016 2,590 1 View
Hi All, I am trying to connect to olive broad institute website to get some genome sequence. But I was not able to access the website. Does anyone know what I did wrong or what's going on with...
09 October 2016 4,898 0 View
Hi All, I'm thinking about to do GBS, but I am not quite sure how good my DNA samples should be, in terms of integrity. I think I have good amount of DNA, but some of my DNA samples may have...
08 September 2016 9,061 6 View
I had 9 pairs of primers added with 4 different M13 tails, two of the 9 pairs primers can not amplify any thing after adding the M13 tails, other 7 pairs worked well. I did not see any small bands...
07 August 2016 5,315 7 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I want to know more about diamond ore deposits in world.
08 August 2024 1,514 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
to identify themes in question with APA style references
07 August 2024 2,239 5 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View