Unequally pooling libraries for RNAseq?

Hi everyone, I have a question about pooling libraries for RNAseq. I have 12 samples that I would like to run RNAseq with. Samples 1-6 were extracted from pure fungal cultures, however, samples...

01 February 2019 3,497 5 View

How to separate oomycete RNA from plant RNA?

Hi all, I was wondering if there is a way to separate oomycete RNA from plant RNA? Any thoughts or suggestions are welcomed. Thanks!

08 September 2018 1,708 0 View

Sas proc glimmix dist=negbin

Hi All, I was trying to analyze some counting data using proc glimmix. I did have a lot of zeros in my counting data, and I specified that the distribution of the counting data follows negative...

05 June 2017 9,885 4 View

How many fungal isolates are needed to present the whole population phenotypically and genotypically in general?

31 December 2016 2,501 1 View

What is the requirement of DNA quality in order to do GBS?

Hi All, I'm thinking about to do GBS, but I am not quite sure how good my DNA samples should be, in terms of integrity. I think I have good amount of DNA, but some of my DNA samples may have...

08 September 2016 8,982 6 View

After adding M13 tail to the forward primer, the pair of primer did not work anymore. Has anyone ever had the same situation?

I had 9 pairs of primers added with 4 different M13 tails, two of the 9 pairs primers can not amplify any thing after adding the M13 tails, other 7 pairs worked well. I did not see any small bands...

07 August 2016 5,243 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View