how to select a dynabead (protein A/G) for Chip experiment? i am aware of invitrogen selectivity chart for this purpose even though experience matters a lot .
Hi,
which Dynabeads to use is very much dependent on species and subclass of the Abs as the Fc part will interact with Prot A or Prot G with different affinity. If the application is e.g immunoprecipitation and the primary Ab is of mouse origin protein G will work well for all IgG subclasses.
kind regards
ketil
Pierce also offers magnetic beads with a combination of A/G. We have had much success with these.
https://www.thermofisher.com/order/catalog/product/88802
hi samantha, thanks for reply
nice to see that product but they have't mention application of these beads in reference to Chip experiment ? and i think that may matter a lot.
have u used this for Chip experiment.
- Badges
- Science topic
- Similar topics
- Publishing