Does anyone know how to screen for significantly different phosphoproteins? I was confused about that screening differential phosphoproteins should be based on phosphopeptides or phosphorylation sites? What's the difference between these two ways? And the significantly different phosphoproteins require normalized by proteome?
Xiaoyin Zeng "When a suitable antibody is available, Western blot is a useful technique to probe for phosphorylation of a specific protein," Chimento says.
The capacity to investigate protein phosphorylation events and abnormal phosphorylation levels is crucial to our knowledge of numerous biological and pathological processes, notably in cancer research. Detecting phosphorylated proteins in western blots, on the other hand, might be difficult.
The EMS-related motif can be created by introducing a negative charge by phosphorylation; this results in reduced SDS binding to the proteins, causing the protein's mobility to be delayed during SDS-PAGE.
Dear Xiaoyin Zeng,
MaxQuant is offering a Summer School every year, whose recordings you can also find on youtube. They have a complete tutorial on how to do PTM analysis of proteins. I am sure you will find your answers there.
Thank you for your suggestion. I will check out the MaxQuant Summer School tutorials on YouTube for learning more about PTM analysis of proteins.@Britta Eggers
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