I would like to ask you a question about sampling the release rate during in vitro digestion of encapsulated lipophilic bioactives. I have performed in vitro digestion of emulsion gel loaded with β-carotene, and during the actual digestion process, the absorbance and cumulative release rate measured at different sampling times did not show a gradual growth trend. Possible reasons: 1. Fat-soluble β-carotene is not easily dissolved in the digestion solution (aqueous phase), resulting in uneven sampling and fluctuating release rate. 2. Can I ask the researchers how to ensure uniformity and reliability of sampling in similar experiments? Can only centrifugation be used to measure the concentration of the supernatant?
Are you sure that your digestion process (which one?) does not attack the lipophilic bioactives (which ones?) as well?
C.A. (Kees) Kan I am a budding student just entering college and I am ecstatic to receive a response from you, Professor.
First of all, thank you for your response. Strictly speaking, I think the gastrointestinal digestion model we are simulating would be somewhat destructive to the bioactives. Using this experiment as an example, different digestion stages of pH, temperature, digestive enzymes, solutes, etc. may destroy the bioactives we have encapsulated. Thus, we may not be able to get perfect data when analyzing the data, but in many mainstream journals and magazines (Food Hydrocolloids, Food Chemistry, International Journal of Biomacromolecules) this in vitro digestion model is followed, and I think this method still has a big error and inaccuracy.
Good that you are aware, that the method may have its restrictions. Still, it might be the best one available at the present time. Do not stop looking for a better one!
C.A. (Kees) Kan You are very respectable, even after so many years of retirement, you are still so active in research gate, you guide our young generation with your profound knowledge and broad vision, and give us valuable advice and encouragement. My highest respect.
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