E-cadherin is single chain glycoprotein, which MW 135KDa,When use 8% gel ,I can't get good-looking band,band is black.So what is the reason could be ? How to deal with this question so that to get good results?
Dear Researchers, I am using the classical Muskhelishvili method to solve plane stress problems in perforated plates with cracks. By introducing a conformal mapping function, the hole boundary is...
03 June 2024 3,185 0 View
Dear researchers, A rectangular steel plate specimen with a central diamond-shaped defect is subjected to uniaxial loading, as shown in the figure. I aim to investigate the mechanisms of crack...
07 May 2024 5,004 1 View
Most current projections of carbon emissions are based on structural data such as the economy, population, and technology. However, unstructured data will also have a certain impact on carbon...
21 September 2023 4,147 1 View
Dear researchers, I am currently researching the issue of fracture in thin steel plates containing holes. Some scholars have already conducted static tensile fracture tests on plates with holes...
04 June 2023 3,549 1 View
There are now many papers in economics that discuss counterfactual prediction. How can machine learning be combined with the field of economics to help counterfactual prediction?
22 May 2023 6,081 3 View
I am currently deriving the stress intensity factor for a crack at the edge of a circular hole in a plate. The integral form of the stress intensity factor is as follows: I would like to inquire...
10 May 2023 6,584 9 View
I am currently studying safe control of power systems and I am interested in finding out whether the security region constraints of power systems can be approximated by a set of linear...
06 May 2023 4,525 3 View
Hi guys, thank you very much for reading my question. To study the phenotype of MDSCs in the tumor microenvironment, I resected the mouse tumor (subcutaneous xenograft), cut it into small pieces,...
05 September 2022 9,406 0 View
The geometric fractal dimension of the solid obtained using the box counting method is generally considered to be the same as that of the pores. For soils where the grain size distribution (GSD)...
22 November 2021 2,283 3 View
I am doing the intracellular staining of IL-22 for intestinal lamina propria cells now. And after staining the cell surface antibody, I stimulated cells with PMA(50ng/ml), ionomycin(750ng/ml), and...
26 September 2019 4,971 0 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View