I am standardizing a latex agglutination test for detecting WSSV with my specific antibody. But agglutination reaction is not evident. I am sure about my antibody; hence, I think it is not properly adhering to the surface of latex beads due to the presence of surfactant in the bead mix. Can anyone help?
Aswathy Chandrababu
You do not write anything about the structure of your surfactant.
You can try to remove the surfactant by washing with ethyl alcohol, but this may remove other molecules. In addition, you
1.Can be removed by converting the hydrophilic group of the surfactant by chemical reaction.
2. Gradual removal of the surfactant monomolecular layer on a Langmuir-Blodgett balance
3. Extraction with various solvents
4. Adsorption on a good adsorbent
5. Adding another surfactant with a charge of the opposite sign and removing the resulting precipitate
The method can be selected after a thorough analysis of the composition, chemical properties of the molecules of your system
polystyrene beads naturally contain negative charges from polymerization reaction from the inbound initiator molecules.
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