How to remove background spots in Elispot particularly when using skin cells in Elispot ?

06 June 2017 0 7K Report

I am using skin cells in EliSpot to detect ASCs but HRP-IgG is pruducing high background in antigen coated wells only not in negative control or positive control (bone marrow) wells.

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

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What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

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