Does learning mathematics exclusively improve cognitive abilities?

Teaching Mathematics at school to all students is commonly justified by the opinion that it improves their problem-solving skills and "makes them smarter" (whichever measure is implied by this). I...

15 February 2021 7,391 7 View

Is there anyone who has and can share journal articles that talk about corrosion test by using the potentiostatic polarization technique ?

I am currently looking for journal articles that explain better the corrosion behavior of steel metal in corrosive medium when using the potentiostatic polarization technique with a plotted graph...

15 December 2020 4,381 7 View

Problems extracting metadata from NCBI in R?

I am trying to extract some information (metadata) from GenBank using the R package "rentrez" and the example I found here https://ajrominger.github.io/2018/05/21/gettingDNA.html. Specifically,...

12 August 2020 5,742 2 View

Peer review standards: should certain consistency be expected?

As an author and a reviewer for several editions I have come across a problem pointed out on many occasions previously, namely the consistency and value of peer-reviews. I have seen good, valuable...

28 June 2020 8,991 8 View

Why do the rentrez and NCBI web searches return different number of hits?

I am querying the GenBank and getting different numbers when compare searches in R (rentrez package) to the Nucleotide db at NCBI website. For example, entrez_search(db="nuccore", term =...

17 June 2020 7,127 3 View

No title

Hi, I generated several machine learning models with different algorithms and hyper-parameters and different preprocesses. My models always end up being systematically biased, as shown in the...

11 March 2020 8,723 2 View

What is the hardness value of austenite and ferrite phase in cold rolled 2205 duplex stainless steel?

I would like to read journal articles where I can find the hardness values of austenite and ferrite phase of the cold rolled 2205 duplex stainless steel.

27 October 2019 9,180 3 View

How can I calculate root mean squared error of cross-validation (leave-one-out) for interpolated data (RMSECVi) ?

Dear Researchers, Anyone can indicate me how to calculate root mean squared error of cross-validation (leave-one-out) for interpolated data (RMSECVi), root mean squared error of...

16 January 2019 7,158 3 View

Why gaussian ended with error termination: no atoms chosen for CPHF?

I am trying to follow Kutateladze protocol for relativistic force field calculation (10.1021/acs.joc.5b00619). In the supporting information, a typical input file is given which I used with very...

16 September 2018 6,784 3 View

Ontology Context to creation JSON-LD serializw format that can be stored in ElasticSearch ?

In order to create a compact JSON-LD serialize form of an Ontology using RDFlib Python Library. I need the given Ontology Context. The compact form is the on that can be stored in ElasticSearch...

10 September 2018 4,597 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View