I have got a stain in my microstructure particularly in ferrite phase which shows now two colour instead of one. So, how can removed the unwanted colour using imageJ software ?
I would like to add an abstract to a book published last year
18 December 2023 6,581 2 View
Sorry for asking probably stupid question, but I got stick at simple application of ISO 2859 sampling plan. Let's take a simple example of sampling according to ISO 2859 as featured on...
02 May 2023 2,340 2 View
Several papers describe a modified DNA isolation protocol with the common DNeasy Blood & Tissue Kit, which introduce an additional step of incubation at 70 °C for 10 min after adding the AL...
01 November 2021 1,356 3 View
Teaching Mathematics at school to all students is commonly justified by the opinion that it improves their problem-solving skills and "makes them smarter" (whichever measure is implied by this). I...
15 February 2021 7,852 7 View
I am currently looking for journal articles that explain better the corrosion behavior of steel metal in corrosive medium when using the potentiostatic polarization technique with a plotted graph...
15 December 2020 4,635 7 View
I am trying to extract some information (metadata) from GenBank using the R package "rentrez" and the example I found here https://ajrominger.github.io/2018/05/21/gettingDNA.html. Specifically,...
12 August 2020 6,067 2 View
Hello everyone, I would appreciate any hints on the identity of this male Mesostigmata. The specimen is badly damaged but looks very peculiar and hopefully can be recognized at least to the...
09 August 2020 6,520 6 View
Good day all, I am working on a paper on illegal logging, for now, I only have data on illegal logging from the Chatham house, I am looking for other data sources to triangulate information to be...
20 July 2020 3,057 0 View
Hello, I did an analysis with MrBayes version 3.2.6 (Ronquist et al., 2012) with the GTR + G + I model with these commands ; END;Begin mrbayes; outgroup polyzona; lset nst = 6 rates =...
07 July 2020 6,631 2 View
As an author and a reviewer for several editions I have come across a problem pointed out on many occasions previously, namely the consistency and value of peer-reviews. I have seen good, valuable...
28 June 2020 9,234 8 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...
29 July 2024 1,981 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View