We tried applying a mere amount of polyimide layer before potting the silica gel, however, we have noticed high level of humidity intervention inside the package.
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
Is there any user-friendly software or R code for haplotype analysis? I run GWAS analysis and now plan to run haplotype analysis. Any suggestions, please?
23 June 2024 4,911 2 View
I am attempting to analyze my data output from the STRUCTURE software to determine the population structure using STRUCTURE Harvester. However, I am experiencing difficulties accessing the...
21 February 2024 2,325 2 View
I'm looking for a dataset that explores Ayurvedic health, including diseases, remedies, and formulations. The data should include information on traditional practices, herbal ingredients,...
09 November 2023 2,488 1 View
Hi, I am working on grid connected PV inverters for high power rating (> 1 MW). Many transmissions companies now requesting the PV farms to support reactive power during night time. I am...
26 September 2023 908 0 View
I have been using a sliding mode observer (as shown in the attachment). I have designed the gains with the assumption that the derivative of the error (between the state estimates and measured...
06 July 2023 5,301 1 View
DMA
12 June 2023 3,960 2 View
how the yoga increasing incretin hormone levels along with mechanisms?
28 May 2023 3,164 3 View
how yoga is affecting the epigenetics and is there any mechanisms available?
19 May 2023 4,114 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...
16 July 2024 7,937 1 View