How to recover DNA bands from gels w/o using a kit
Hi everybody, We want to replace our old imaging system for qualitative DNA/Agarose Gels (working on Win 3.11!!!) and I wander if it would be useful to switch from EtBr to CybrGreen. How does...
02 March 2009 6,376 1 View
Hi, Getting hints to solve one's problems is one of the main things on ResGATE. This indeed is fine but normally the community is not informed whether or not the information helped to resolve the...
01 February 2009 1,131 2 View
Hi everyone, I'd like to perform whole RNA amplification of minute samples. Anybody experienced in this technique? What about biased amplification (underrepresentation of low expressed mRNAs)?
31 December 2008 7,020 7 View
Hi, here are some links I found quite helpful when I had to design PCRs UCSC In-silico-PCR (http://genome.ucsc.edu/cgi-bin/hgPcr?command=start) This site allows you to retrieve DNA...
31 December 2008 9,667 0 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View
