How to recover DNA bands from gels w/o using a kit
Hi everybody, We want to replace our old imaging system for qualitative DNA/Agarose Gels (working on Win 3.11!!!) and I wander if it would be useful to switch from EtBr to CybrGreen. How does...
02 March 2009 6,298 1 View
Hi, Getting hints to solve one's problems is one of the main things on ResGATE. This indeed is fine but normally the community is not informed whether or not the information helped to resolve the...
01 February 2009 1,057 2 View
Hi everyone, I'd like to perform whole RNA amplification of minute samples. Anybody experienced in this technique? What about biased amplification (underrepresentation of low expressed mRNAs)?
31 December 2008 6,933 7 View
Hi, here are some links I found quite helpful when I had to design PCRs UCSC In-silico-PCR (http://genome.ucsc.edu/cgi-bin/hgPcr?command=start) This site allows you to retrieve DNA...
31 December 2008 9,586 0 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...
20 February 2021 6,897 1 View
I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...
15 February 2021 7,444 2 View
I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...
14 February 2021 7,800 7 View
I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...
09 February 2021 8,594 3 View
In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...
08 February 2021 2,792 1 View
Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...
07 February 2021 9,530 3 View
I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...
04 February 2021 1,509 3 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View
