Does anyone know about the method to quantify incotine by HPLC-DAD?
Never heard of "incotine". What is that? Perhaps you meant to write, nicotine instead?
If so, that is very easy to retain on a C18 column with a gradient of acidified water vs ACN and UV/VIS detection. Sample prep may or may not be more involved, depending on the source. *A keyword search on the web will turn up hundreds of free HPLC methods for such a common compound.
Sorry, that you mention is correct, I will follow with your suggestion by HPLC-DAD on a C18 column with a gradient of ammonium acetate buffer and methanol, but when I search about it, there are many method to try about nicotine analysis. My project is to quantify nicotine in e-cigarette.
Yes, this is very easy to run. *I often start beginners off with this method because it is so simple to set up and run a full calibration. Many free methods can be found on the web. Select the one that is most applicable to your needs or start with the suggestion that I made earlier.
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