How to purify mono-BOC-ethylenediamine or N-tert-butoxycarbonylaminoethylamine (EDA-BOC) by Column cromatography? I used ethylacetate (100% ) over silica gel to purify but it does not work. EDA-BOC either dicomposed or stuck in silica gel. Some one told me to use triethylamine with mobile phase but I am not sure about it. I have sythesized EDA-BOC by following Luminescence 2011; 26: 264–270, doi 10.1002/bio.1223 but still geting some impurities. Anyone suggest me best way to purify EDA-BOC with appropiate reference?
Purify mono-BOC-ethylenediamine or N-tert-butoxycarbonylaminoethylamine by Column chromatography, you can try chloroform methanol 95:5 % by volume as an eluting solvent. first see separation on TLC , then go to column.
Thank you. What do your recommend for stationary phase ? Silica ?
First check TLC carefully by using different normal solvent (like chloroform, methanol, ethyl acetate, diethyl ether, hexane) with different ratio (5:1, 10: 1, 50:1, 100:1, ), do column with the appropriate ratio taking silica as stationary phase. And then think about other solvents......
Thanks..Its not a general chemistry ..I need expert advice those who work on it for long time..
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