Currently, I would like to confirm the ratio of live/dead pathogen(bacteria) in urine patient sample using dye. However, there are a lot of other cells (including WBC, RBC, Epithelial cell and etc.), thus it is very difficult to figure out the ratio live/dead bacteria. So I think that if I could purify bacteria only from urine patient sample, staining will be working. How can I purify bacteria only from urine patient sample?
Sol Choi
Matthew Ibbs Thanks for kind reply. I would like to figure out the ratio of live/dead of bacteria(pathogen) in urine sample. (I think that the question as I mentioned above is unclear) and it should be achieved in hour (avoid growth-based screening). How can I use the flow cytometry?
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