How to purify and extract heat shock proteins HSP70 from blood cattle ?

Hassan Nima @Hassan_Nima2

01 January 2018 1 4K Report

What is the best way to extract and purify heat shock proteins HSP70 from blood of cattle ?

Jamie Nicole Tedeschi

Hi Hassan,

Have you seen this paper by Basiricò et al. 2011?

doi: 10.1007/s12192-011-0257-7

Hopefully that can help!

Regards,

Jamie

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Hassan Nima's questions See All

How to convert a Text File to a Fasta Format File?

10 November 2018 3,936 0 View

Which came first, the German Peasants War or the English Peasants Revolt?

German Peasants War, English Peasants Revolt

05 June 2018 7,031 1 View

Why the DNA considered a genetic material ?

DNA , genetic material

04 May 2018 9,780 0 View

Which is the most credible free online IQ test?

credible free online IQ test

04 May 2018 3,605 1 View

Are there any animals that actually smile when they're happy ?

animals that actually smile when they're happy

04 May 2018 9,762 0 View

Is an offline IQ test more reliable than a online IQ test?

offline IQ , online IQ test

04 May 2018 2,624 1 View

At the age of 28, is it too late to start a PhD in the biotechnology field or other ?

the age of 28, start a PhD

03 April 2018 3,887 98 View

What is the best way to extract DNA from fungi ?

extract DNA, fungi

02 March 2018 4,916 3 View

If the bacteria multiply all their genes within 36,000 minutes, how many genes have multiply within one hour ?

gene multiply

02 March 2018 4,777 0 View

Staying in space for a long time affects DNA or gene expression ?

space , DNA, gene expression

02 March 2018 6,631 0 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View