i am trying to figure out how to propagate bacteriophage- phi 6.
i try different media (NBY, 2XYT, LB) and different tempearture (22-28oC)to propagate as mention in different article.
i am worried about the bacteriophage-phi 6 stability as well may be its not stable.
anyone having a experience to propagate RNA bacteriophages that might be helpful in my research.
need your suggestion thanks in advance
Hi Muhammad, any solution to your problem? I have the same challenge now.
Thanks
Marjan
sorry, i tried multiple times but did not find any solution and so I quit this project. later working on that project I find multiple phages from the environment. these phages have the same host as phi-6
Thanks. let me know if you learn anything new on phi6 propagation, Im still struggling with it.
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