we study the bioremediation lead (lead nitrate and lead chloride) by bacterial, how to prevent the deposition of lead solution in broth agar after centrifuge?
Beast regards
It seemingly may help to sequester lead(II) with some suitable chelating agent, such as: citrate, dimercaprol (BAL), 2,3-dimercaptopropanol, ethylenediaminetetraacetic acid (EDTA), D-penicillamine, or tartrate.
It depends in what do you want to do and what problems are you experiencing.
If it is to measure the final Pb concentration, Perhaps you don't need to use centrifuge if you use other types of separation (as the one mentioned by Carlos Araujo).
if it is to prepare you experiment, maybe you want to consider Pb Kps in your solution.
hope it helps
As said above, use a chelating agent. In a chloride- and/or phosphate-rich circumneutral media, you are going to have this problem unless you use a chelator. But this also affects to the general chemistry of the system...
You may want to try an alternative to centrifugation. But that depends on what you are trying to accomplish.
Maybe of some interest ― while considering PbCl2 precipitation: https://www.researchgate.net/post/How_to_obtain_a_PbCl2_water_solution
Maybe of some interest ― while considering PbSO4 precipitation: https://www.researchgate.net/post/How_do_I_dissolve_PbSO4_powder_in_distilled_water
hello
this link could help
http://pubs.acs.org/doi/abs/10.1021/cm801269g
regards
I also suggest as references:
J.L. Pauley, M.K. Testerman, "Basic salts of lead nitrate formed in aqueous media", J. Am. Chem. Soc., 176(16) 1954, 4220–4222;
G. Todd, E. Parry, "Character of lead hydroxide and basic lead carbonate", Nature, vol. 202, 1964 (25 April), 386–387.
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