Hello everyone
I am going to fabricate a hydrogel using PEG-SH via a thiol-en reaction. It is well known that PEG-Thiol is prone to oxidation, and it has been suggested in the literature that it would be better if one could use a glove box or deoxygenated water.
unfortunately, I don,t have access to the equipment to do so,
I wanted to know if there are any alternative ways to make this hydrogel efficiently.
Is using a reducing agent applicable for this? Does,t it interfere with the formation of hydrogel?
I would appreciate any recommendations.
Thanks
Dear Maryam Alizadeh, you can use a Shlank setup. For further details, please google search the following book, you may find a free to download copy. My Regards
The Manipulation of Air–Sensitive Compounds
I have worked with thiolated hyaluronic acid, which has a thiol group. Generally, oxidation of the free thiol group to disulphide bond occurs at basic conditions(> 7). Under acidic conditions, it is stable. For thoil-ene reaction, if you are using PBS 7.4 then dissolve your PEG-SH at low temperature (5 °C) and then elevate the temperature to RT only before mixing with -ene.
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