Calibration curve methodology from APHA
What is your dependant variable?
Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...
16 February 2021 8,389 3 View
I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...
09 February 2021 9,012 3 View
Hello everyone. I am about to search for the expression of genes including p53 in methylated and non methylated HPV samples. Should I extract DNA from the methylated samples and use SYBR green...
04 February 2021 2,749 3 View
Instead of appearing one single correlation of the signal of the carbon at 20.9 ppm (#11 in the attached figure), aligned to hydrogen at 2.29 ppm, it appeared two correlations (2,32 and 2,26 ppm)....
16 January 2021 4,774 2 View
I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...
06 January 2021 7,218 2 View
I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2...
28 December 2020 4,426 6 View
I have four HPLC graphs (for methyl ethyl ketone (MEK) and sulfuric acid and little organic monomers) and I want to analyze them. Is there anyone who can help me with this?
23 December 2020 6,180 3 View
I am looking for an online tool to see whether a certain methylated CpG site can affect the expression of a certain gene or not?
18 December 2020 7,575 1 View
Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in...
09 December 2020 8,832 4 View
I am trying to predict age from DNA methylation. I am using conventional PCR master mix, but I didn't get my PCR product. Is it due to Master Mix? Should I use HotStar Taq Master Mix ?
08 December 2020 8,776 4 View
