I want to prepare galacturonic acid oligomers with 5-8 repeating units. How should I do this? Is it possible to prepare it from pectin hydrolysis? How?
Make it from monomer with suitable catalyst/enzyme but with a limited amount of monomer.
could you explain how should I do it? what is the suitable catalyst/enzyme?
Dear F Seidi , I have no conditions for this particular oligomer, but remember in the past the solution of a similar problem, done that way, regards.
Here you are
Preparation of oligomers of galacturonic acid from pectic acid
Oligogalacturonides were prepared by autolysis according to a modified procedure of (Robertsen, 1986). Briefly, 5 g of commercial pectic acid (Aldrich) were dissolved in 500 ml of distilled water, and the pH was adjusted to 4.2. The suspension was autoclaved (20 min, 121°C), and the pH was then adjusted to 2.0. After incubation at 4°C (16 h), precipitated larger oligomers were removed by centrifugation (20 min, 4000 g). The supernatant was adjusted to pH 5.0 and applied to a DEAE-Sephadex column (2 cm diameter×42 cm). Oligomers were slowly eluted with a linear 2-L-gradient of ammonium acetate (0–1 M, pH 5.2), and fractions (8 ml ) were tested for the presence of sugars using the phenol/sulphuric acid assay (Dubois et al., 1956). Fractions containing the dimer to hexamer were pooled separately and freeze-dried several times. Purity and identity of oligomers were confirmed by co-elution with known standards (characterized by liquid secondary ion mass spectrometry (LSI-MS) and 1H-NMR-spectroscopy (NMR) ) upon high-pH-anion exchange chromatography (HPAEC) (Maness et al., 1990).
---Small oligomers of galacturonic acid are endogenous suppressors of disease resistance reactions in wheat leaves, Bruno M. Moerschbacher, Manfred Mierau, Barbara Graeßner, Ulrike Noll and Andrew J. Mort, Journal of Experimental Botany, Vol. 50, No. 334, pp. 605–612, May 1999.
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