I have a confusion wether should i use the animal manure directly into the AD process or should I have to do something else before adding it into the digester with substrate?
You can use animal manure directly in the anaerobic digester (AD).
Please ensure Volatile Solids (VS) of the Substrate = 0.5 X Volatile solids (VS) of inoculum (in your case its animal manure). This is valid for batch-fed AD.
I agree with Imad to start by removing all inert solids from the manure (cow dung) without exposing it to the open air (oxygen being toxic for methanogens) as we did back in 1988 to seed/inoculate our largest UASB in India at Jubilant Organosys. At that time, cow dung was the only inoculum available at sufficient quantities.
Awais Aqib you have to consider the total solids content along with the organic dry matter
or dry matter and organic dry matter and based on the OLR of the digester just add water and homogenize it with water and ensure proper slurry is made and then feed it to the digester based on the design.
One can ensure that after feeding for 20-25 days without disturbing it the gas generation can be seen and further one can take the next course of action based on the feedstock the digester is designed for
if this is designed for animal manure then start feeding slowly and gradually increase the amount to the designed capacity
I hope you are doing fine. The anaerobic digestion process would fail if you do not provide the necessary conditions needed. By this I mean both the feedstock and the digester must have specific properties to generate the highest amount of biogas possible. You should make sure that the feedstock entering the digester contains enough amount of water, which could be up to 70% of the total feed. Furthermore, you may preheat the feed before pouring it into the digester if the process would be done in thermophilic and high temperature conditions (up to 60 centigrade degrees). Although this step is not necessary, but it is highly recommended as it could preheat the feed and could result in more amount of biogas. During the digestion process, you should make sure the PH of the digester content would be as constant as possible and would not be lower/higher than 4.5/6.8. You may also stir the content on a regular basis if you have a continuous AD digester.