Hi all,
I have synthesized a metal nanoparticle incorporated membrane catalyst. I wanted to analyze the same by HRTEM. Could anybody please suggest a method to prepare the grid for this kind of membrane sample for HRTEM analysis?
Dear Ansari,
first you need TEM grids available from different suppliers. Then you have to make a dilute suspension of your sample in an inert solvent (depends on your sample). Then add one drop onto the grid inside of some glassware and dry the grid by vacuum or temperature (whatever fits your sample best). Drying is especially important beacuse of contamination in TEM, so do not use to high vacuum pressures/too low temperatures
Hope this helps
Thank you Mr. Lucas. Is there any other way to prepare the grid so that i can maintain the membrane structure as such.
Dear Ansari,
I am not sure what you mean with membrane structure, but if heating is no option for preparation, try using high vacuum alone. But you have to exclude (carbon) contamination from TEM. Otherwise the operator will be angry because usage of TEM will be delayed only by this contamination.
You could also try freeze-drying.
The most sophisticated method would by kryo-TEM, but I do not know about preparation for these
Hope this helps
Lucas Warmuth
- Badges
- Science topic