Dear Friends,
I am working on a R&D project about increasing alkaloid content of Papaver sp. in in vitro conditions. My project manager asks me to isolate codeine from total alkaloids extracts from Papaver culture. Since I have no opinion about the procedure I checked online procedures and only find out cold water extraction way which is not suitable. I have to isolate the final product as pure as possible. Do you have any suggestions about it?
Thanks for your kindly and helpful replies.
Hello Deniz,
I am working on alkaloids, but haven't worked on codeine specifically, I'm not familiar with it. However, I can give you afew suggestions.
- Cold extraction may work and still give you alkaloids
-Since alkaloids are non-polar, use non-polar solvents to extract. May consider 100% or gradients of the following, n-Hexane and DCM, or gradients of the two with ethyl acetate.
-Purify your fractions and isolate on HPLC
-Next step has two options depending on the availability of your resources:
- Either use a pure codeine if you have it as standard on HPLC or TLC and check which of your purified isolates match with the curve areas or RF of the standard codeine,
- Or, you can check these (Area under curve or RFs) online for pure codeine and compare with your fractions'.
- The one that matches is it. The tricky part is on the isolation, be very patient and sure on this stage.
All the best
The following links may help, though some of them not from plant material:
https://www.researchgate.net/publication/303971773_%27Cold_water_extraction%27_of_codeine_A_method_of_tampering_with_%27over-the-counter%27_analgesics
https://www.linkedin.com/pulse/nice-extraction-method-identify-codeine-hydrocodone-cough-benny-lum
https://www.unodc.org/unodc/en/data-and-analysis/bulletin/bulletin_1950-01-01_3_page004.html
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658028/
By the way, soxhlet method can also help if you do not wish to do cold extraction. Keep it
Thanks for suggestions. I have checked most of them. I was planning to optimize calculations on analytical HPLC and perform extraction on preperative HPLC. Some collegues are suggested me to use FPLC for higher amount of sample (Like 20 lt last product to purify). What should i do?
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