Most FTIR software usually allows you to upload and overlay the two data files. Once loaded, select the software function which can be used to normalize the 'Y' scales used. Normalize the scales using a key peak, not associated with any part of your sample to insure correct scaling.

Dear Ishan Vyas

If you measured your samples in the form of pellets with the same amount of sample in KBr in each pellet, then you should NOT normalize, as the required information (total amount of polysaccharide in the sample) is present in the intensity of the polysaccharide bands. If you would use normalization, then you would get the same absorbance for all samples.

Alternatively, you can use an internal standard and then normalize the spectra using a suitable band of this standard - choose a band that does not overlap with the bands of you polymer.

Regards

Josef

Ishan...Any comparison needs the use of almost identical sample preparation steps including the quantitative parameters like sample size and KBr amount and after grinding the amount of the mixture to make the disc. The instrumental settings need also to be equalized. The spectrum of the various sample, therefore, will have a baseline almost identical and any comparison will be useful and manageable. However the purity of samples is a determining factor to give the required resolution. Even normalization software will necessitate the identical conditions or at least known quantitative data.

Hi

Ftir spectral guides are available in net. You can compare yiur peaks with the references and ascertain the molecules.

Best