How to more accurately extract the area of interest in adult rat brain?

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Sample size calculation for multinomial logistic regression model?

May I know how to do Sample size calculation for multinomial logistic regression model?

10 November 2018 9,690 3 View

How to standardize coefficient in multinomial logistic regression model in SPSS?

(1). How to standardize coefficient in multinomial logistic regression model in SPSS? (2). After standardization of the coefficients in different multinomial logistic regression model, can we...

10 November 2018 7,648 0 View

How long can i put my sciatic nerve tissue in the formalin solution for future immunofluroscent staining?

how long can i put my sciatic nerve tissue in the formalin solution for future immunofluroscent staining?

04 May 2018 265 0 View

How to quantify axonal loss without taking nerve biopsy but take nearby tissue in carpal tunnel syndrome?

I plan to take some tissues nearby while doing open release for carpal tunnel syndrome to quantify axonal loss of the injured median nerve. May I know any good laboratory techniques ? Thank you

01 February 2018 9,282 1 View

How to check multicollinearity of IVs in ordinal regression model using SPSS?

Dependent Variable (DV) is ordinal data. Independent variables (IVs) are continuous data. Using SPSS, Multicollinearity test can be checked in linear regression model. However, this is required...

11 December 2017 4,795 4 View

How to deal with irrational but statistically significant predictor in the model?

I have a dilemma when doing bootstrapping method to examine my model: I identified predictor A & B in my regression model as significant estimates. However, it can be rational only when (A...

10 November 2017 7,975 9 View

How to determine accuracy of predictors in ordinary regression model using SPSS ?

I have created an ordinary regression model with acceptable model-of-fitness, significant predictors, no sig. of test-of-paralelle line etc. Now I wish to determine accuracy of my predictors of...

10 November 2017 7,364 9 View

How is the performance of the ROC curve acceptable for publication?

I have sort out cut-off values of variables for differential diagnosis using an assessment tool. Although all the cut-off values are significant, however, the area under the curve (AUC) are from...

08 September 2017 9,555 2 View

How to perform multinomial scaling using SPSS?

Any guidelines for performing multinomial scaling using SPSS?A step-by-step guideline is preferred.

01 January 1970 8,023 0 View

With a poor performance in ROC curve of the model, does that mean the model is useless at all?

I created a binary model with results as follow: 1). Omnibus Test (Chi-sq.=31.705, p0.05) The model was statistically...

01 January 1970 3,875 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Enrico Marani

Contact E.A.J.F. Lakke on the LUMC, he made a kind of cortex overview of the rat.

Leiden University Medical center. Good luck. Marani

Xinxin Ge

There are some atlas that could potentially help you to align cortical regions. You may also consider stimulating the hindpaw (try not to have hindlimb involved) and use the responding area as your ROI.