i wanna modify a vector according to my requirement while taking that vector as a base and modify that.
gibson assembly and classic restriction/ligation cloning are a good starting point.
What are the modern approaches to the organization and sustainable management of greenery in urbanized cities? How can ecological and recreational functions be introduced into the landscape and...
23 July 2024 7,292 4 View
Please suggest how to identify the herbs, shrubs and trees in field
25 March 2024 7,172 6 View
For DNA islolation
13 March 2024 7,635 4 View
Hi, We need to buy a laser that is centered at 700-800 nm range (780 nm for example), has high output power (>100 mW), tunable (at least couple of nanometers), and has a linewidth preferably...
27 February 2024 9,548 2 View
70SiO2
15 January 2024 8,304 0 View
Currently, I am working on a project, and I want to study the marine ecotoxicity of rust. When CS pipe is exposed to the marine environment during the usage phase, it begins to degrade, resulting...
17 December 2023 7,734 4 View
After 3 hours of exposure of formic acid under UV in the presence of BaWO4 and metallic silver and metallic nickel in the presence of BaWO4 and metallic silver and metallic nickel, I observed an...
05 December 2023 3,169 0 View
For example, It may help in Mortality reduction in mass, detection and conservation of the nursery grounds etc.
24 September 2023 3,824 0 View
I have completed a master's degree in computer science and have good research experience in the human-computer interface area. I have published 3 IF journals as a first author and 7 IEEE...
15 September 2023 2,778 1 View
The optimal distance between source and receiver for detecting laser ultrasonic reflections from delamination was determined. Time of flights (TOF) was estimated for shear wave. Could you please...
12 August 2023 6,827 0 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...
22 June 2024 6,165 2 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
Should we use special binary vectors when using plasmid rescue method to detect transgene integration site in plant? that is, there is often no ori site between LB and RB region of simple binary...
28 May 2024 1,873 0 View