12 December 2018 8 6K Report

Hi everyone,

I am a master student in medicine.

in the paper " Late Ca2+ Sparks and Ripples During the Systolic Ca2+ Transient in Heart Muscle Cells", I dont understand how to measure the distance from T-tubule to Late calcium spark (figure 1D). is there anyone can explain more detail about the figure 2D in this article for me please?

Thank you for taking time resolving my issues.

and this is the context:

" A simple, obvious, explanation for the genesis of the LCSs would be that they arise from jSR that was either not activated during the upstroke of the Ca2+ transient or was uncoupled or orphaned17 from t-tubules (which carry the AP to the cell interior). To examine this idea, we labeled t-tubules (Figure 1D) and measured the Euclidian distance to the LCS centroid (Figure 1E) and compared the latencies for the upstroke of the Ca2+ transient at LCS sites to overall Ca2+ transient latency (Figure 1F). Perhaps unexpectedly, LCS occurred close to t-tubules and at positions where the Ca2+ transient developed with a shorter than average latency (P

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