How to measure Elasticity by using Force-Volume AFM curves?

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Nicolas F. Martinez

Dear Sonia,

Tipically the Force Volume AFM curves should be sufficient to do the calculations. Which version of Nanoscope Analysis and AFM are you using?. Also, which material are you measuring and more importantly, what is the spring constant of the tip you are using for elasticity measurements?.

There is also the possibility to use free software like AtomicJ to do the calcultations.

Sonia Bagheri

Thank you Nicolas for your response. I am using Nanoscope analysis v1.5 and AFM Bruker multimode 8 (Nanoscope 9.1) for the experiments. In Nanoscope software, just Hertzian and Sneddon models exist. My material is collagen, which I am testing in the air in PF QNM mode using scanasyst-air (Bruker) cantilever with the spring constant of 0.4 N/m.

I didn't know about AtomicJ software. thank you for suggesting