How to determine the cell density or total suspended solids of the bacteria which are entrapped in hydrogel like PVA+Alginate?
Hi,
I hope that these articles will be useful. Best Luck.
Article Isolated Reporter Bacteria in Supramolecular Hydrogel Microwell Arrays
Article Metabolic Responses of Bacterial Cells to Immobilization
You can read those articles online.
Article Rheology of peptide- and protein-based physical hydrogels: A...
https://www.ncbi.nlm.nih.gov/pubmed/25266637?dopt=Abstract
Article Control of Three-Dimensional Cell Adhesion by the Chirality ...
Article Micropatterning of Hydrogels by Soft Embossing
Thank you Badreddine, Midhtak and Traci for the suggestions! Will try out these methods and see which one work the best for my experiment.
Hello, MTT assay to estimate viable cell density of bacteria entrapped in hydrogel may be used, however a proper braking of the hydrogel and proper extraction by propanol and following centrifugation will be needed. For more information please see the paper Article A cryogel-based bioreactor for water treatment applications
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