I observed discoloration of dye in the presence of nanomaterials in the dark. production of ROS in the dark was also observed. How we can prove the degradation is due to adsorption or catalytic degradation.
Hi Lakshmi,
You can confirm that in various ways.
First, Before taking the absorbance, you centrifuge the solution at high speed and take the upper portion carefully. From the spectra, you will confirm that it is due to the catalytic degradation not because of dye adsorption.
In a second method, you can carry out one experiment where one solution will be exposed with light and other one in the dark, record the UV-Vis at the same time interval. If both the spectra overlap with each other, means its due to adsorption not catalytic degradation.
Finally, you characterize the NPs after finishing the experiments. That will tell you whether dye adsorption takes place or not.
Hi, In addition you should also look for quenching of absorbance or fluorescence of dyes in the presence of nanoparticles /nanomaterials.
You can carry out one experiment where one solution will be exposed with light and other one in the dark, record the UV-Vis at the same time interval. If both the spectra overlap with each other, means its due to adsorption not catalytic degradation.
Finally, you characterize the NPs after finishing the experiments. That will tell you whether dye adsorption takes place or not.
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