Dear ,
I want to make MS containing Acetosyringone for Agrobacterium to infect explants (Chinese cabbage)?
SO when I make MS solution, do I still need to sucrose? If sucrose is needed , what concentration(%) do I need to use?
I just prepared MS medium (without changing ingredients of MS medium), then added Acetosyringone into cool-down MS medium, and then mixed them well.
Yes, you need to add sucrose in the MS medium (probably 3% should be fine). and as suggested by Yuan-Yeu Yau above, once the medium has been prepared, adjust PH., autoclave and when the medium is of bearable warmth you can add ACS, mix well.
I will also need to prepare B5 containing acetosyringone. I have red an article maybe it can help you.
Article An efficient Agrobacterium-mediated transformation method us...
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