The effect of chealant addition including EDTA, DTPA, EDDS,and citric acid on phytoremdiation of metals within different organic contents of soil has been investigated .......Chelating agents have been shown to desorb heavy metals from the soil matrix into the soil solution, and to facilitate metal translocation from root to shoots. The effect may vary from plant and metal species. Chelating agents are frequently been shown to assist phytoremediation. To prepare the different concentrations of EDTA and Citric acid You should first prepare sufficient 0.1 EDTA stock solution and take aliquots nof it to dilute it to needed concentration. For more details consult Journal of Environmental & Analytical Toxicology........
To prepare 0.1 M citric acid:
Dissolve 19.21 g of anhydrous citric acid in 1 L of distilled water.
To prepare 1 M solution of EDTA (ethylenediaminetetraacetic acid) is not possible, because water solubility of EDTA is 0.5 g.L-1 at 20 o C. 1 M EDTA solution should have an EDTA concentration 292.2 g per L.
Regards
Vit
Thank you for your suggestion Vit Mateju Sir.How can we prepare solution of 1M EDTA for treatment.
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