01 January 2015 1 4K Report

I'm trying to load an AM-ester dye in an acute hippocampal brain slice. I am specifically interested in microglia (which are GFP labelled), however, I understand that microglia are particularly difficult to image with these dyes. 

We recover for 1 hour in aCSF at room temperature after slicing, then I will incubate with the dye (containing DMSO and diluted in aCSF) at room temperature for 30 min. 

Does anyone have tips for getting AM-ester dyes into microglia?

Thanks!

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