I'm trying to load an AM-ester dye in an acute hippocampal brain slice. I am specifically interested in microglia (which are GFP labelled), however, I understand that microglia are particularly difficult to image with these dyes.
We recover for 1 hour in aCSF at room temperature after slicing, then I will incubate with the dye (containing DMSO and diluted in aCSF) at room temperature for 30 min.
Does anyone have tips for getting AM-ester dyes into microglia?
Thanks!
This RP might lead you to the exactly needed references.
The use of calcium-dependent fluorescent indicator dyes has enabled the measurement of synchronized activity across a network of cells. This technique gives both high spatial resolution and sufficient temporal sampling to record spontaneous activity of the developing network.
J Vis Exp. 2011; (56): 3550.
Published online 2011 Oct 22. doi: 10.3791/3550
PMCID: PMC3227196
Functional Calcium Imaging in Developing Cortical Networks
Julia Dawitz et al
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