Growth-associated protein 43 (GAP43) is well known marker of neurite outgrowth. In our recently published paper we have shown that 10 days after cortical injury in the neurons of injured cortex high density of GAP43 staining was concentrated in a particular part of the neuronal cell body forming growth-cone-like structures, which were directed to the lesion site.

Brkic et al. Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats

Brain Injury (2012) 26 (10):1273-1284,

Dear colleague, maybe you can use a polyclonal antibody for panclonal neurofilament for a picture of total neurons; and monoclonal antibody vs neurofilament 200 for new axons. In this sense you can obtain a general idea from movement of new neurons in neural tissue environment. I hope this opinion could help you in the practicing, good look

Thanks a lot- I will try with phalloidin and/or Vangl2. Do you have any suggestion about any more specific antibody ?

Dear Collegue

you can try anti-GAP-43

Thanks. It´s works also in adult rats?

Yes

I tried GAP-43 immunostaining for identification of sprouting without so much success... By chance, if you have antibody that works on adult rats, I would be interested to have the reference if you don't mind.

Dear Maxime .As well as you, in our experience GAP 43 doesn't works in adult rats.

I tried GAP-43 immunostaining for adlult rat sections but it does not work in my case too

In this case we still have a problem. Any more idea ?

Several antibodies available that recognise intracellular 2G13P which is localized to Growth Cones,particularly filopodia and lamellipodies in developing rat CNS

Thanks a lot. Do you belive that also will work well in adults?

Don't see why not. You are after all developing new CNS

Nice idea, I like it..I am working in a cortex repair animal model (see reprint). I will try with 2G13P. If you have any more suggestions will be most than wellcome

Thank you for your answer too! Would you think that it's a good marker for sprouting?

If 2G13P could be a sprouting marker, I would like to have the reference details so that I can try for my samples too. Thanks in advance.

Original reference

JOURNAL OF NEUROCYTOLOGY

Volume 28, Number 12 (1999), 1035-1044, DOI: 10.1023/A:1007044207002

Monoclonal antibody 2G13, a new axonal growth cone marker

Olivier Stettler, Maxwell S. Bush, Melissa Kasper, Burkhard Schlosshauer and Phillip R. Gordon-Weeks

No idea about sprouting marker

Dear Duncan, your paper will be very useful for me. Thank you very much indeed.

Dear Dr. Malleswari, We will be in touch.. My e mail address is [email protected]. Regards

Growth-associated protein 43 (GAP43) is well known marker of neurite outgrowth. In our recently published paper we have shown that 10 days after cortical injury in the neurons of injured cortex high density of GAP43 staining was concentrated in a particular part of the neuronal cell body forming growth-cone-like structures, which were directed to the lesion site.

Brkic et al. Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats

Brain Injury (2012) 26 (10):1273-1284,

These findings confirm my idea that survival time is important in lesion plasticity!. Probably I will come back on GAP43. Thanks a lot.

Dr. Pekovic - Could you be so kind to send to me by e mail a reprint of your paper?

[email protected]. Thanks in advance

Dear Dr Pekovic, I would also be interested in a pdf version of your paper if you don't mind? [email protected]

Thanks in advance

Thanks for your answer.