I need to remove most of the proteins from urine samples to see how background noise contribtues to signal intensity in LFA.
I tried pH treating and heat treating and it did not work! I still had so much protein! I had extra antibodies to try to immunoprecipitate out but it did not work either.
I also thought about a column.
What do you think would denature and kill off proteins the best?
Dear Sir. Concerning your issue about how to remove most of the proteins from urine samples. I think the following below link may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211279/pdf/jbt.14-2504-004.pdf
Thanks
Hi there,
I would suggest ultrafiltration on low MW cut off membrane.
you can use both heat treatment with a high concentration of an acid like HCl to break the peptide bonds. It might be helpful
so while its heated, you want me to add a high con. strong acid? Will I blow up? lol
Yes you will blow up and your problem will be totally solved :)
No you can cook it at 100 Celcius with 6N HCI
It is called hydrolyse :-P
First time I tried it, it there was still so much He4 left. Didn't make a dent in the concentration.
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