How to isoalate DNA from fecal samples

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I am using LB/circle grow to grow bacterial cells and isolate plasmid dna. I am looking for an easy to prepare in-house media. Can any one suggest?

Though I am using either LB broth or circle grow media to grow bacterial cells, I am looking for high cell density easy to prepare media rather than procuring commercially. Can any one suggest me.

06 July 2018 3,829 0 View

Is there any method to detect mutations caused by Taq Polymerase with less proofreading capacity and low fidelity?

I have used a Taq Polymerase with less Proof reading and fidelity. I have a doubt that some mutation may be occurred during the PCR. One drawback is the polymerase doesn't have 3'-5' exonuclease...

04 May 2015 8,631 4 View

I am facing problems when I clone my DNA in freedom p CHO vector either at the AVR II/BST site or the ECO RV/ PAC I site. Can any one help me?

Hi all, I am working on freedom p CHO vector. There are two restriction sites called AVR II/ Bst Z171 and ECO RV/Pac I. I am able to digest my vector at the sites and I am able to clone at any one...

02 March 2015 2,146 4 View

Can anyone guide me in learning next generation sequencing software?

Is Biolinux enough?

03 April 2014 9,136 2 View

How to run the BEAST software?

Can anybody explain how to run the BEAST software for bayesian analysis of molecular sequences. Thank you.

08 September 2013 6,803 1 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

Has anyone used or is familiar with an anti-C4 complement factor antibody for Western Blotting?

Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...

17 June 2024 6,124 1 View

Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

13 May 2024 3,061 11 View

Why does soybean half-seed transformation use 1/10x B5 salts during co-cultivation?

I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...

07 May 2024 3,924 0 View

Why I am not getting accumulated GFP in vacuole for om45 WT GFP yeast strains?

I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...

04 May 2024 6,463 0 View

What are the genetic mechanisms governing the production of secondary metabolites in microorganisms?

How are these exploited in biotechnological applications?

07 April 2024 566 5 View