I am using LB/circle grow to grow bacterial cells and isolate plasmid dna. I am looking for an easy to prepare in-house media. Can any one suggest?

Though I am using either LB broth or circle grow media to grow bacterial cells, I am looking for high cell density easy to prepare media rather than procuring commercially. Can any one suggest me.

06 July 2018 3,732 0 View

Is there any method to detect mutations caused by Taq Polymerase with less proofreading capacity and low fidelity?

I have used a Taq Polymerase with less Proof reading and fidelity. I have a doubt that some mutation may be occurred during the PCR. One drawback is the polymerase doesn't have 3'-5' exonuclease...

04 May 2015 8,494 4 View

I am facing problems when I clone my DNA in freedom p CHO vector either at the AVR II/BST site or the ECO RV/ PAC I site. Can any one help me?

Hi all, I am working on freedom p CHO vector. There are two restriction sites called AVR II/ Bst Z171 and ECO RV/Pac I. I am able to digest my vector at the sites and I am able to clone at any one...

02 March 2015 1,986 4 View

Can anyone guide me in learning next generation sequencing software?

Is Biolinux enough?

03 April 2014 9,040 2 View

How to run the BEAST software?

Can anybody explain how to run the BEAST software for bayesian analysis of molecular sequences. Thank you.

08 September 2013 6,699 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View