Kesav Kumar Bheemavarapu

6 Questions 7 Answers 0 Followers

Questions related from Kesav Kumar Bheemavarapu

I am using LB/circle grow to grow bacterial cells and isolate plasmid dna. I am looking for an easy to prepare in-house media. Can any one suggest?

Though I am using either LB broth or circle grow media to grow bacterial cells, I am looking for high cell density easy to prepare media rather than procuring commercially. Can any one suggest me.

07 July 2018 3,798 0 View

Is there any method to detect mutations caused by Taq Polymerase with less proofreading capacity and low fidelity?

I have used a Taq Polymerase with less Proof reading and fidelity. I have a doubt that some mutation may be occurred during the PCR. One drawback is the polymerase doesn't have 3'-5' exonuclease...

05 May 2015 8,594 4 View

I am facing problems when I clone my DNA in freedom p CHO vector either at the AVR II/BST site or the ECO RV/ PAC I site. Can any one help me?

Hi all, I am working on freedom p CHO vector. There are two restriction sites called AVR II/ Bst Z171 and ECO RV/Pac I. I am able to digest my vector at the sites and I am able to clone at any one...

03 March 2015 2,107 4 View

Can anyone guide me in learning next generation sequencing software?

Is Biolinux enough?

04 April 2014 9,105 2 View

How to run the BEAST software?

Can anybody explain how to run the BEAST software for bayesian analysis of molecular sequences. Thank you.

09 September 2013 6,762 1 View

How to isoalate DNA from fecal samples

It is very difficult to isolate DNA from fecal samples, can you share the problems and its troubleshooting.

11 November 2011 3,677 3 View