I know the technique which is sub-culturing the bacterial treated with sub-MIC for the specific days like 30 days. I am writing if anyone knows another technique to investigate this?
You can probably set up a smaller version of this (though very similar to the sub-MIC method):
https://www.youtube.com/watch?v=plVk4NVIUh8
Very cool experiment, and you can pick up all the colonies that 'break' the antibiotic barrier and perform WGS on them and compare them to the parent clone to see what caused the resistance to occur.
To my mind, the experimental technique (using of sub-MIC of antibacterial in recultivation series), which You know is good and accurate one.
You can probably set up a smaller version of this (though very similar to the sub-MIC method):
https://www.youtube.com/watch?v=plVk4NVIUh8
Very cool experiment, and you can pick up all the colonies that 'break' the antibiotic barrier and perform WGS on them and compare them to the parent clone to see what caused the resistance to occur.
Hello Laleh
Its important to investigate the bacterial sensitivity to antibacterials, screening for resistance vectors such as plasmids and sometimes phages or transposons also may be sufficient but we preferring the screening the drug resistance genes directly...
Hi
I like this approach
https://www.aaas.org/news/scientists-build-giant-petri-dish-film-bacteria-resistance
best regards
Dear Khodaparast,
You can go for "Antimicrobial interactions: mechanisms and implications for drug discovery and resistance evolution" published in Current Opinion in Microbiology (2015) for some exciting knowlegde in your field.
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