Is it possible to have a leaky expression of a mutant protein while the wild-type protein's is inducible?

i did a site-directed mutagenesis of HBx gen in pET-16b plasmid to produce HBx T118N. The HBx wild-type overproduction in e.coli BL21(DE3) required IPTG 0,1 mM. On the other hand, HBx T118N could...

15 May 2018 551 3 View

How to model a CFD domain with density gradient ?

Hello everyone, I'm interested in simulating water discharge pipe inside a lake. But I have some problem, the lake water has a certain density gradient profile. How to model the density gradient...

08 October 2017 4,875 5 View

How to determine geometric coordinate of a minimum length nozzle using Method of Characteristic?

I've done calculation to define the Characteristic lines as guided by J.D Anderson's book "Modern Compressible flow" but I can't find the plausible algoritm or method to determine the geometry....

10 May 2017 5,406 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View