These bacteria are usually saprophytic (environmental) and are not pathogenic, but are now considered one of the most frequently found opportunistic microorganisms in nosocomial infections (Hospitals) in immunosuppressed patients. The one that is part of a pathology depends more on the host than on the strain itself, although as this bacterium acquires mechanisms of resistance very easily and can usually become multiresistant strains. This latter characteristic represents an additional risk since it is difficult to treat and eradicate it. But there is no system to identify non-pathogenic pathogens, now if you can identify the pattern of resistance to antimicrobials to know how sensitive or resistant is to the antibiotics commonly used by the method of Kirby and Bauer and that in turn Makes it clear whether the bacterium is acquired in the community (wild strain generally very sensitive) or in the hospital environment (generally multiresistant strain). This bacterium is usually very aggressive in patients with cystic fibrosis but by the very condition of the host.

For identification in genus and species

Planting and insulation

For isolation, MacConkey or cetrimide agar can be used, incubated at 30 ° C (environmental isolates) or 37 ° C (nosocomial isolates) for periods of 24 hours. Non-fermenting colonies on MacConkey agar are inoculated into Kligler's medium to check the non-fermenter characteristic. Gram-negative rods should be observed microscopically.

Conventional Methods

Preliminary characterization

For the identification of BGNNF at the genus and species level, the use of morphological and biochemical tests is essential, including: Gram staining, motility, oxidase, growth at 42 ° C, seeding in OF basal medium supplemented with 1% The following carbohydrates: glucose, maltose, mannitol, xylose, fructose and lactose in the basal medium OF (Oxidation - fermentation), and a wide range of complementary tests such as determination of arginine dehydrolase, lysine decarboxylase, hydrolysis of esculin and gelatin. Susceptibility test for polymyxin B (300 U).

Semi-automated and automated methods such as API System and Micro Scan System can also be used.

Identification of Pseudomonas species is currently performed by using molecular methods such as the comparison of 16S rRNA sequences or the rRNA-DNA hybridization although, until now, the rpoB gene is considered the most suitable for the identification and phylogenetic discrimination at a species and subspecies level.

I hope I've helped

But which is specific test to differeciate?

Hello dear. Pseudomonades, are ubiquitous and widespread in nature, inhabiting soil, water, plants, and animals.Many species are saprophytic or pathogenic for plants. The fluorescent group of Pseudomonas comprises P. aeruginosa, P. fluorescens, P. putida , ... are  opportunistic human pathogens that cause disease in immunocompromised hosts and individuals with cystic fibrosis. When pyoverdin combines with the blue,

water-soluble phenazine pigment pyocyanin, the bright green color characteristic of P. aeruginosa is created (P.a is most important sp)

In addition, T3SS (Ttpe III secretion system), Phospholipases, QS and ... are other virulence factors that are present in all of P.aeruginosa.

MDR isolate of P.aeruginosa such as ESBLs are more important than other isolates. 

for detection of ESBLs you can detect of sensitivity of the isolates to the third-generation cephalosporins (ceftazidime, cefotaxime, ceftriaxone, Third generation cephalosporins (3GC), 30 µg each) was determined by disc diffusion method using P. aeruginosa ATCC 27853 as control strain

Any reference for this?

Dear Sohel S Shaikh

Pls. find the attached files

http://peer-reviewedjournals.com/articles/JMB-MS12-2-03.pdf

http://jb.asm.org/content/190/8/2858.full

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.1966.tb03499.x/full

http://www.cell.com/cell/abstract/S0092-8674(00)80958-7?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0092867400809587%3Fshowall%3Dtrue

http://www.cell.com/cell/abstract/S0092-8674(00)81724-9?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0092867400817249%3Fshowall%3Dtrue

https://www.quora.com/What-is-the-difference-between-coliform-and-non-coliform-bacteria

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686621/

https://www.jstor.org/stable/42882869

http://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-392

Hoping this will be helpful

Regards

Prof. Houda Kawas

123

Hi Sohels

You can test the pathogenicity

You can see this reference:

"Avirulent and Nonpathogenic Pseudomonas Species"

https://www.apsnet.org/publications/phytopathology/backissues/Documents/1996Articles/Phyto86n07_757.PDF

Thanks for your answers

Hello, these are the identification tables of the most frequent species of the genus Pseudomonas, I hope they serve you.

Marielsa 

I want the test to differenciate between pathoenic and non pathogenic species.

All strains may become pathogenic or not at any given time because it will depend on the host's immune system. However, there is an increased risk of those presenting acquired resistance mechanisms, such as those that produce Betalactamasas type AmpC or also those that produce Betalactamasas (BLEE) or those producing metalo-betalactamases (MBLs) in Pseudomonas aeruginosa. AmpC betalactamases, BLEE y MBLS are associated with high mortality and, at a laboratory level, with false antimicrobial susceptibility reports. Therefore, its routine investigation is vitally important in microbiology laboratories. In the links that I am going to place are the techniques to detect phenotypically these strains with these mechanisms of resistance. I hope that the Spanish language is not a problem. 

http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S1315-25562009000200003

http://cybertesis.unmsm.edu.pe/xmlui/bitstream/handle/cybertesis/243/Diaz_tj.pdf?sequence=1

http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0075-52222012000200002

Thanks for your answers

You most welcome

Houda

Iagree with Marielsa Gil