How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How to get the inflow and outflow values for conducting Life Cycle Assessment (LCA) of Concrete and geopolymer concrete?

Are the inputs and outputs required for conducting LCA of concrete is taken from literature or we have any database like ecoinvent that provide us with the data (inflow and outflow of processes)...

23 November 2020 8,832 3 View

The most of Physicists, who worked in the field of Statistical physics, committed suicide. Is statistical physics really a killer subject?

Tragically, in 1906, Boltzmann committed suicide and many believe that the statistical mechanics was the cause. He provided the current definition of entropy, interpreted as a measure of...

19 October 2020 9,482 18 View

How are the black holes connected to the creation of galaxies?

Congratulation to 2020 Physics Laureates (Roger Penrose, Reinhard Genzel, and Andrea Ghez for their discoveries about black holes). Penrose, Genzel, and Ghez explained to the world the black holes...

05 October 2020 5,313 9 View

My protein is Cadmium binding protein. Can I use Cadmium column instead of Nickel column for purification of protein via IMAC method?

I want to do structural analysis of the protein after purification.

19 February 2019 3,656 6 View

How to get a stable/intact double stranded RNA (dsRNA) after treatment with RNase A?

My research objective is to silent a targeted gene through RNA interference (RNAi). I cloned my designed gene fragment (double stranded RNA) in L4440 vector under the control of two...

24 May 2018 9,968 5 View

My research will be Pharmaceutical Process validation on solid dosage form drugs.I need to write research proposal and so I want to know how to do it?

my research project is on Pharmaceutical Process Validation during tablet manufacturing and I want to know the techniques, methods, ways, and materials required for it. Any papers related to it?

27 February 2018 1,575 6 View

Current List of Journals on Thomas and reuters?

Does anyone know the current list of Thomas and reuters Journals.

30 January 2018 4,691 2 View

What should be the appropriate vehicle to dissolve L-DOPA?

I want to dissolve L-DOPA to treat my melanoma cells. I tried with DMSO, PBS and ethanol, but L-DOPA did not dissolve in those solvents. Then I tried with nuclease free water which dissolve L-DOPA...

30 November 2017 895 3 View

How xanthine oxidase inhibitor might influence the renin: angiotensin system?

Iam interested in the mechanism of xanthine oxidase inhibitors

06 July 2017 6,134 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View