Alternative method of Permeablization in IL17A assay by flowcytometry?

I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the...

01 December 2020 4,553 1 View

Suggestions how to get Shapefile of India with all 700+ Indian districts?

I need shapefile for all 700+ districts of India. From where I can find the India shapefile with all districts. Also, suggest how to get the latest India shapefile with all state and union...

07 August 2020 3,449 5 View

PSO, ABC or GA, which one to choose while solving Mixed Integer Programming problem?

I am interested to solve a mathematical problem (MILP) using evolutionary algorithms but confuse about which one to choose as a beginner in the programming languages. Suggest an algorithm easy to...

13 July 2020 4,027 51 View

What is the best tool for adding references (in a research paper) within a word file?

What is the best tool for adding references (in a research paper) within a word file

16 June 2020 8,275 9 View

How can I check the viability of RAPD Primers?

Dear Researchers Good day to you I want to ask if we can check our RAPD primers ( both in diluted & lyophilized forms) on agarose gel electrophoresis whether they are degraded or not as our...

24 February 2020 9,172 3 View

How can I develop an ANFIS model for WQI in matlab?

I want to implement the Water quality index in ANFIS. I have used constant output, but not getting the desired output.

06 February 2020 3,394 4 View

Can food supply chains be secured by blockchain diffusion???

Blockchain technology promises to provide complete information security and elimination of intermediary alteration. But can we really full utilize this feature to food supply chain. How can we...

03 May 2019 5,476 2 View

When the unit of analysis is 'supply chain' or 'organization', is it acceptable to take more than one respondent from an organization?

Question related to research methodology

06 March 2019 9,150 7 View

No title

02 May 2018 368 2 View

How to generate UDF in Fluent to measure Droplet concentration at a particular downstream in Turbulent flow?

I don't know how to write UDF. If you know then Please help me..or you can also write the UDE for me and Send me.

22 February 2018 1,883 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View

Is it possible to get compass potential paramters from MATERIALS STUDIO 6.0?

Hi all, I wonder whether we can get potential parameters from material studio. I have a licensed version available. If I use polymers and cnt in materials studio to perform MD simulations,...

03 February 2021 7,673 3 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View