Cramp because too Happy, sad or tired?

Hi. I have a friend who Will getting cramp if he was too Happy, sad, or tired. Does anybody know, whar is the diesease might he got? Thank you

31 December 2018 1,865 0 View

Found Isovaleric Acid in my Nata de Coco Product?

Hi, i'm found isovaleric acid in my final nata de coco product. Does anybody know where it come from? Does it cause by microbe or some chemist reaction? Please give me an explanation if anybody...

11 December 2018 1,797 3 View

Why does it mean when Primer-dimer occur on negative control in ARMS PCR?

I am currently working on ARMS PCR and the result is there is primer dimer in every negative control that i made. I was doing optimatization and there are pirmer dimer in every samples using...

10 November 2017 5,331 1 View

Found Isovaleric Acid in my Nata de Coco Product

Hi, i'm found isovaleric acid in my final nata de coco product. Does anybody know where it come from? Does it cause by microbe or some chemist reaction? Please give me an explanation if anybody...

01 January 1970 2,279 6 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View